Signalling

Part:BBa_I13015:Design

Designed by: Debra, Fred, Jay, Madeleine, Cell-Cell Signaling SMUG   Group: Antiquity   (2004-07-08)


3OC6HSL Sender controlled by pBad


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1873
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


Design Notes

The pictures below are from a time course, where pRCV-3 produced GFP via HSL-bound LuxR promotion. I13015 was on the left half of the slide (induced in .1% arabinose, thereby producing the HSL for pRCV-3), while pRCV-3 was on the right half.

Controls were done with pRCV-3 alone, I13015 alone and the two together without any arabinose. In all three cases little or no GFP was expressed.


Source

References